LiNK2©
The LiNK2© is an easy-to-use, convenient and disposable no-power device that collects and purifies biological environmental samples in the field.  It is designed to collect liquid and dry biological samples and quickly and conveniently prepares them for PCR analysis. 

The convenience of LiNK2© allows a collected sample to be processed immediately and/or returned to its dessicant-filled shipping container and store at room temperature with no degradation of the DNA.  Its dependable accuracy takes the guess work out of every application.

It was designed, developed, tested and certified with end-user input and reactions!


LiNK2© features an unprecedented package:
  • Quick, accurate on-site analysis & feedback
  • Lightweight & conveniently portable
  • Works with liquids, slurries & surfaces
  • Multifunctional for sample collection/preservation/archiving
  • Economical, disposable container device
  • Ease of operation, even with HazMat gear
  • No specialized training required
  • Minimal operational functions
  • Field ready - no power, vacuum or toxic reagents required
  • No toxic, organic or flammable components
  • Adaptable to a variety of lab & field PCR instruments
  • Compatible with high throughput/automation in lab settings
  • Shipped QA/QC certified
  • Pre-packaged, ready to use with purified PCR Water
  • Available in both irradiated and non-sterilized configurations
  • Additional Syringes and Cannula Tips available


The Future Is Today!
The LiNK2© has been created to accommodate most standard PCR units and has been proven to be the most highly respected choice for sensitive and specific identification of unknown biological substances.  Historically, however, there has been no fast, easy and economical method for sample preparation that enhances on-the-spot PCR analysis --- until LiNK2©.

First responders need tools that make rapid, reliable and accurate threat assessments resulting in an informed decision.  They need to discover the elements they face --- and quickly.

Does the element pose a health threat?  Is it a hoax?  Is it a simple misunderstanding? What is the solution?

The best PCR machine will not work with inhibited samples, and most samples such as environmental, clinical, food, etc., contain PCR inhibitors.  The LiNK2© solution changes that by providing economical and time-saving enhancements that have been developed under the scrutiny of PCR technologists and manufacturers.  The LiNK2© changes the parameters of traditional PCR applications.


T R I A R I I
S c i e n t i f i c,  L L C
1.Collect sample
  onto paper end
  of LiNK body

= Elution
  Buffer
= Biological
  Warfare 
  Agent
= PCR
  Inhibitor
2.Wait 5min. for
  LiNK paper to
  lyse agent,
  bind, some 
  PCR inhibitors

3.Insert LiNK body
  into elution tub filled
  with elution buffer

4.Push firmly to
  fully engage LiNK
  body in tub

5.Aspirate eluate into
  syringe. Release
  and remove syringe
  from LiNK body

6.Inject sample into
  PCR machine

DNA
= DNA
The LiNK2© Solution – Quick, Easy and Accurate!
LiNK2© operates easily, effectively and economically, opening the door to a broad range of governmental, civil and commercial users that have been previously inhibited by the absence of extensive laboratories, timing and budgets.  Some of these users include:

  • EM First Responders
  • Fire Departments
  • Police Departments
  • HazMat Teams
  • Airport Security
  • Port Security
  • Postal Services
  • Building/Facilities Owners/Managers
  • Federal/Government Agencies
  • Military/Defense Teams
  • Food Industries
  • Bio-Chemical Facilities
  • Facilities that cater to large audiences
  • Manufacturing Facilities Dealing with Bio-Materials

Detection Limits for LiNK-processed Samples in Fieldable and Lab-Based Systems for B.Anthracis and B.Thuringiensis
(Test Results from MIT Lincoln Laboratory and from the Naval Medical Research Center (NMRC))

Independent tests have proven that PCRs using the LiNK2© collection solution performs the biological threat analysis at a comparable level as standard laboratory facilities and processes.
(Sensitivities achieved using laboratory techniques) See chart below
PCR System    Sampling Matrix
Culture Media   Waste-water          Sand
  RAZOR                103 CFU/mL (103)          103 CFU/mL (102)     <102 CFU/g (<102)
LightCycler           102 to 103 CFU/mL (103)    103 CFU/mL (102)     <102 CFU/g (<102)
AB7900               >103 CFU/mL (103)       >103 CFU/mL (102)          >102 CFU/g
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LiNK2 fits easily
in your hand
The LiNK2 Solution
Collection of the Biochem Agent